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KMID : 0923620170170020103
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Immune Network
2017 Volume.17 No. 2 p.103 ~ p.109
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Detection of Autoantibodies against Aquaporin-1 in the Sera of Patients with Primary Sjogren's Syndrome
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Alam Jehan
Choi Yun-Sik
Koh Jung-Hee
Kwok Seung-Ki
Park Sung-Hwan
Song Yeong-Wook
Park Kyung-pyo
Choi Young-Nim
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Abstract
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The pathophysiology of glandular dysfunction in Sjogren's syndrome (SS) has not been fully elucidated. Previously, we reported the presence of autoantibodies to AQP-5 in patients with SS, which was associated with a low resting salivary flow. The purpose of this study was to investigate the presence of anti-AQP1 autoantibodies. To detect anti-AQP1 autoantibodies, cell-based indirect immunofluorescence assay was developed using MDCK cells that overexpressed human AQP1. By screening 112 SS and 52 control sera, anti-AQP1 autoantibodies were detected in 27.7% of the SS but in none of the control sera. Interestingly, the sera that were positive for anti-AQP1 autoantibodies also contained anti-AQP5 autoantibodies in the previous study. Different from anti-AQP5 autoantibodies, the presence of anti-AQP1 autoantibodies was not associated with the salivary flow rate. Although anti-AQP1 autoantibodies are not useful as a diagnostic marker, the presence of autoantibodies to AQP1 may be an obstacle to AQP1 gene therapy for SS.
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KEYWORD
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Sjogren's syndrome, Aquaporin 1, Autoantibodies, Fluorescent antibody technique
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